Hindi
  • Sunday | 22-October-2017

Microbiology

Microbiology

Dr V P Myneedu Head of the Department View Profile
Dr Manpreet Bhalla Senior Research Officer (SAG) View Profile
Dr Ajoy Verma  Specialist View Profile
Dr Ritu Singhal Specialist View Profile
Dr Niti Singh NRL Microbiologist View Profile
Dr Jyoti Arora NRL Microbiologist View Profile
Dr Gavish Kumar FIND Microbiologist View Profile

List of Equipments

Biological Safety Cabinet ESCO Labculture Class II, NuAire class I biological safety cabinets, GeneXpert System, BACTEC™ MGIT™ 960, Rota 6R-V/Fm refrigerated centrifuge, Grant Inspissator, Vertical autoclave, Refrigerated Centrifuges, Kahn’s shaker, Incubator, Walk in incubator, Walk in cold room, Vortex mixer, Deep freezers, Refrigerator, Vortex Mixer, GT blot 48 Line Probe Assay machine, PCR machine, twin-cubator, hot air oven, PCR hoods.

This year Microscan rapid bacterial identification system and ELISA reader and washer have been added.

Activities

The Microbiology Department of LRSI is an advanced centre for TB with facilities from microscopy to molecular tests. The Department has a distinction of being one of the National Reference Laboratories (NRL) under Revised National TB Control Programmewith the Central TB division. As NRL, the Department conducts training for the Microbiologist and Lab Technician of the Intermediate Reference Laboratories (IRL) of the allotted states, accreditation of various IRLs.The Department is also involved in training of the postgraduate students, lab technicians, MedicalM.Sc and Medical B.Sc for dissertation and internship

The various sections of Department are.

MYCOBACTERIOLOGY

With staff of 1 Microbiologist (Specialist), 1 microbiologist (NRL), 2 senior technicians, 5 seniors TB lab technicians (NRL), the section performs smear microscopy, processing of samples for culture and drug sensitivity for first line and second line anti-tubercular drugs by 1% proportion and absolute concentration method. The conventional culture system has been certified by SNRL Antwerp Belgium for first and second line drugs sensitivity testing. The facility has conducted successful certification of culture & DST laboratories in Haryana, AIIMS (Medicine), NDTB centre, Department of Microbiology, Chandigarh

LIQUID CULTURE SYSTEM (MYCOBACTERIOLOGY)

Established in 1994, currently the section is staffed with 1 Specialist Microbiologist, 1 microbiologist (NRL), 1 microbiologist (FIND) and 2 technicians. The section comprises 2 BACTEC MGIT 960 fully automated machines. The section performs smear microscopy, processing of samples, culture and drug sensitivity for first line and second line anti-tubercular drugs by liquid culture for patients (MGIT). The section has been certified by SNRL Antwerp Belgium for first and second line drugs. The section has involved in certification of culture & DST laboratories of NDTB centre, Department of Medicine, AIIMS and Department of Microbiology, Chandigarh.

MOLECULAR DIAGNOSIS (MYCOBACTERIOLOGY) LINE PROBE ASSAY

Started in October 2011, with staff of 1 Specialist Microbiologist and 4 technicians (FIND) , LPA facility has the strength to conduct DNA extraction, amplification and hybridization of over 40 samples per day. Samples are received from LRS-DR-TB centre (five districts of Delhi) and also from states of Punjab, Sikkim and North Eastern states till their local Labs achieve their capabilities. The facility has conducted successful certification of LPA laboratories in Haryana, Chandigarh and Meghalaya.

NON-TUBERCULOUS MYCOBACTERIA IDENTIFICATIONSYSTEM

The identification of all Mycobacteria are routinely done till the species level by conventional method using various biochemical tests and rapid immune-chromatographic test. The section is in process of developing molecular tests for identification of NTMs

BACTERIOLOGY UNIT

The section performs identification and drug susceptibility for all bacteria. The section has now fully automated bacterial identification and sensitivity system (MICROSCAN)

MYCOLOGY

The section performs routine KOH microscopy, india ink preparation and fungal isolation ( Moulds and yeast) as per standard guidelines. Aspergillus serology is done by gel-precipitation method and now ELISA has been started. There is collaboration with Mycology Department of Patel Chest Institute for confirmation of Aspergillus isolates by molecular methods and susceptibility.

SEROLOGY

Tests are conducted for HIV, HBsAg, HCV and Typhoid (Widal)

 

PARASITOLOGY

Stool examination for ova/ cyst using standard guidelines is performed routinely.

Ongoing Research Projects

  1. Pilot study for isolation and identification of Mycobacterium avium in HIV patients.
  2. Use of Bactec MGIT 960 Automated System for recovery of Mycobacteria from Extra pulmonary Specimens and typing of isolates using Spoligotyping.
  3. Prevalence of Multi- drug resistant Mycobacterium tuberculosis in India using line probe assay; National Reference Laboratory.
  4. Molecular epidemiology and Genetic characterization of M. tuberculosis from HIV seronegative and HIV seropositive patients funded by ICMR, New Delhi.
  5. To study the feasibility of determining multi-drug resistance among M. tuberculosis using colorimetric liquid culture method in comparison with 1% proportion method.
  6. Detection of Mycobacterium tuberculosis from gastric aspirates in pediatric patients from LRSI using polymerase chain reaction (PCR) and conventional culture and their comparison”.
  7. Species Identification of Non Tuberculous mycobacteria from patients with pulmonary and extra pulmonary tuberculosis.
  8. Study of Air Quality in the Wards OPD & Labs of LRSI (A Mycobacteriological Study).
  9. Molecular analysis of MDR and XDR strains of M.tuberculosis isolates form a tertiary care hospital.

Publications:

  1. Jyoti Arora, Manpreet Bhalla, Zeeshan Sidiq, Prabha Lal, Digamber Behera, Nalin Rastogi, V.P. Myneedu. Predominance of Beijing genotype in Extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi India. International journal of Mycobacteriology. April 2013 (Indexed).
  2. Ritu Singhal, Manpreet Bhalla, Surjeet Chakraborty, Niti Singh, Digamber Behera, V.P. MyneeduPhenol-ammonium sulfate microscopy method for diagnosis of tuberculosis.Accepted for publication in international journal of Mycobacteriology. April 2013 (Indexed).
  3. Ritu Singhal, Jyoti Arora, Prabha Lal ,Manpreet Bhalla, Digamber Behera, Vithal Prasad Myneedu, Comparison of Line Probe assay with liquid culture for detection of multi-drug resistance in Mycobacterium tuberculosis. Indian Journal of Medical Research December2012;6,136:1044-1047 (impact Factor: 2.0).
  4. Singhal R, Arora J, Bhalla M, ShakirMd, Visalakshi P, Behera D, Myneedu VP Presumptive identification of Mycobacterium tuberculosis complex based on cord formation in Bactec MGIT 960 medium. 2012; 30 : 218-221(Impact factor- 1.006).
  5. JyotiArora, RituSinghal, Manpreet Bhalla, Shakir Reza, PotharajuVisalakshi, DigamberBehera and Vithal Prasad Myneedu.Diagnostic Utility of Capilia TB Assay for Identification of Mycobacterium tuberculosis Complex. Current Research in Tuberculosis.2012;04:13-18.
  6. PotharajuVisalakshi, Sandeep K. Meharwal, JyotiArora, Manpreet Bhalla, Niti Singh, Vithal Prasad Myneedu and DigamberBehera. Fate of sputum samples transported in bottles containing cetylpyridinium chloride and sodium chloride: a national reference laboratory study- Short communication. Indian Journal Tuberculosis 2012;59: 112-115.
  7. Siddiqi S, Ahmed A, Asif S, Behera D, Javaid M, Jani J, Jyoti A, Mahatre R, Mahto D, Richter E, Rodrigues C, Visalakshi P, Rüsch-GerdesS.Direct drug susceptibility testing of Mycobacterium tuberculosis for rapid detection of multidrug resistance using the Bactec MGIT 960 system: a multicenter study. J ClinMicrobiol. 2012 Feb;50(2):435-40 (Impact factor- 4.22).
  8. V.P.Myneedu, P.Visalakshi, Verma AK, BeheraD,Bhalla M. Prevalence of XDR-TB cases - a retrospective fact finding from a tertiary care TB hospital. Indian J Tuberc 2011; 58:54-59.
  9. VP Myneedu,AKVerma, PP Sharma, D Behera A Pilot Study of Same Day Sputum Smear Examination,Its Feasibility and Usefulness in Diagnosis of Pulmonary TB. Indian J Tuberc 2011;58:160-167.
  10. H.Syre, V.P Myneedu, V.K. Arora, and H.M.S. Grewal Direct Detection of Mycobacterial Species in Pulmonary Specimens by Two Rapid Amplifiaction Test, the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test and the Geno Type Mycobacteria Direct Test. J.Clin.Microbiol.2009 Nov., 47(11):36359.Epub2009Sep 30.
  11. PotharajuVisalakshi, JyotiArora, DigamberBehera. Evaluation of Nitrate Reductase. Assay for Rapid Detection of Resistance to Second-Line Drugs in Mycobacterium tuberculosis in a Tertiary Care Hospital. DiagnMicrobiol Infect Dis. 2010 Nov; 68(3):236-40. Epub 2010 Sep 20. (Impact factor- 2.380).
  12. Peptide –Based Antibody detection for Tuberculosis Diagnosis J. Clinical and Vaccine Immunology. Jan 2009 page, 49-54 vol. 16 no.-1. GuomiaoShen, DigamberBehera, Manpreet Bhalla, Arthur Nadas and SumanLaal. (Impact Factor:2.47 ).
  13. VisalakshiP, Meharwal S, Myneedu V.P, Behera D (2009) Evaluation of direct method of drug . susceptibility testing of Mycobacterium tuberculosis to rifampicin and isoniazid by nitrate reductase assay in a national reference laboratory Diagonistic Microbiology and Infectious Disease 66:148-152.
  14. Ajay Wanchu., Yuxin Dong., Sunil Sethi, V.P. Myneedu, Arthur Nadas, Zhentong Liu, John Belisle, SumanLaal A. Biomaekers for Clinical and Incipient Tuberculosis: Performance in a TB-Endemic Country. PLoS One.2008 Apr 30; 3 (4):e2071.
  15. Simplified detection of Mycobacterium Tuberculosis in sputum using smear Microscopy and PCR with Molecular beacon. Journal of Medical Microbiology. October 2007 56,1356-1362.Sagarika Haldar,SoumiteshChakravorty, Manpreet Bhalla, Shyamasree De Majumdar and Jaya SivaswamiTyagi (Impact Factor:2.38.
  16. Detection of acid fast bacilli in post lysis debris of clinical specimens improves the reliability of PCR – Journal of Clinical Microbiology. July 2005 P3580-3581. SagarikaHaldar, Shyamasree De Majumdar, SoumiteshChakravorty, Jaya SivaswamiTyagi, Manpreet Bhalla and M. K. Sen. (Impact Factor: 4.2).